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progrp isoform 3 (aa 1–115+8)  (OriGene)


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    Structured Review

    OriGene progrp isoform 3 (aa 1–115+8)
    Progrp Isoform 3 (Aa 1–115+8), supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/progrp+isoform+3/progrp+isoform+1/pm24518900-29-29-39
    Average 90 stars, based on 1 article reviews
    progrp isoform 3 (aa 1–115+8) - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Purification:

    Article Title: Determining ProGRP and isoforms in lung and thyroid cancer patient samples: comparing an MS method with a routine clinical immunoassay
    Article Snippet: This paper compares two methods to determine the tumor marker progastrin-releasing peptide (ProGRP): as routine assay, the automated time-resolved immunofluorometric assay (TR-IFMA), which allows total ProGRP determination; and the immunocapture liquid chromatography selected reaction monitoring mass spectrometry (LC–SRM-MS) method, which additionally allows isoform differentiation.. The investigation included 60 serum samples from patients suffering from various cancer diseases which may cause elevated ProGRP levels (small cell lung carcinoma; SCLC, non-small cell lung carcinoma; NCLC; and medullary thyroid cancer; MTC, as well as some with unspecific diagnosis).. The two methods showed good correlation (R=0.887).

    Article Title: Digging deeper into the field of the small cell lung cancer tumor marker ProGRP: a method for differentiation of its isoforms.
    Article Snippet: TPCK-treated lyophilized trypsin from bovine pancreas was sequencing grade (Sigma-Aldrich). .. Recombinant ProGRP products, short ProGRP (AA 31−98), ProGRP isoform 1 (AA 1−125 + 8), ProGRP isoform 2 (AA 1−141 + 8), and ProGRP isoform 3 (AA 1−138 + 8), were cloned from human cDNA (OriGene Technologies), expressed in Escherichia coli (Promega) using pGEX-6P-3 constructs (GE Healthcare) and purified as described elsewhere.20 Concentrations of the ProGRP solutions were determined by absorbance at 280 nm (A280) using individually calculated extinction coefficients. ..

    Recombinant:

    Article Title: Determining ProGRP and isoforms in lung and thyroid cancer patient samples: comparing an MS method with a routine clinical immunoassay
    Article Snippet: This paper compares two methods to determine the tumor marker progastrin-releasing peptide (ProGRP): as routine assay, the automated time-resolved immunofluorometric assay (TR-IFMA), which allows total ProGRP determination; and the immunocapture liquid chromatography selected reaction monitoring mass spectrometry (LC–SRM-MS) method, which additionally allows isoform differentiation.. The investigation included 60 serum samples from patients suffering from various cancer diseases which may cause elevated ProGRP levels (small cell lung carcinoma; SCLC, non-small cell lung carcinoma; NCLC; and medullary thyroid cancer; MTC, as well as some with unspecific diagnosis).. The two methods showed good correlation (R=0.887).

    Article Title: Digging deeper into the field of the small cell lung cancer tumor marker ProGRP: a method for differentiation of its isoforms.
    Article Snippet: TPCK-treated lyophilized trypsin from bovine pancreas was sequencing grade (Sigma-Aldrich). .. Recombinant ProGRP products, short ProGRP (AA 31−98), ProGRP isoform 1 (AA 1−125 + 8), ProGRP isoform 2 (AA 1−141 + 8), and ProGRP isoform 3 (AA 1−138 + 8), were cloned from human cDNA (OriGene Technologies), expressed in Escherichia coli (Promega) using pGEX-6P-3 constructs (GE Healthcare) and purified as described elsewhere.20 Concentrations of the ProGRP solutions were determined by absorbance at 280 nm (A280) using individually calculated extinction coefficients. ..

    Clone Assay:

    Article Title: Determining ProGRP and isoforms in lung and thyroid cancer patient samples: comparing an MS method with a routine clinical immunoassay
    Article Snippet: This paper compares two methods to determine the tumor marker progastrin-releasing peptide (ProGRP): as routine assay, the automated time-resolved immunofluorometric assay (TR-IFMA), which allows total ProGRP determination; and the immunocapture liquid chromatography selected reaction monitoring mass spectrometry (LC–SRM-MS) method, which additionally allows isoform differentiation.. The investigation included 60 serum samples from patients suffering from various cancer diseases which may cause elevated ProGRP levels (small cell lung carcinoma; SCLC, non-small cell lung carcinoma; NCLC; and medullary thyroid cancer; MTC, as well as some with unspecific diagnosis).. The two methods showed good correlation (R=0.887).

    Article Title: Digging deeper into the field of the small cell lung cancer tumor marker ProGRP: a method for differentiation of its isoforms.
    Article Snippet: TPCK-treated lyophilized trypsin from bovine pancreas was sequencing grade (Sigma-Aldrich). .. Recombinant ProGRP products, short ProGRP (AA 31−98), ProGRP isoform 1 (AA 1−125 + 8), ProGRP isoform 2 (AA 1−141 + 8), and ProGRP isoform 3 (AA 1−138 + 8), were cloned from human cDNA (OriGene Technologies), expressed in Escherichia coli (Promega) using pGEX-6P-3 constructs (GE Healthcare) and purified as described elsewhere.20 Concentrations of the ProGRP solutions were determined by absorbance at 280 nm (A280) using individually calculated extinction coefficients. ..

    Construct:

    Article Title: Determining ProGRP and isoforms in lung and thyroid cancer patient samples: comparing an MS method with a routine clinical immunoassay
    Article Snippet: This paper compares two methods to determine the tumor marker progastrin-releasing peptide (ProGRP): as routine assay, the automated time-resolved immunofluorometric assay (TR-IFMA), which allows total ProGRP determination; and the immunocapture liquid chromatography selected reaction monitoring mass spectrometry (LC–SRM-MS) method, which additionally allows isoform differentiation.. The investigation included 60 serum samples from patients suffering from various cancer diseases which may cause elevated ProGRP levels (small cell lung carcinoma; SCLC, non-small cell lung carcinoma; NCLC; and medullary thyroid cancer; MTC, as well as some with unspecific diagnosis).. The two methods showed good correlation (R=0.887).

    Article Title: Digging deeper into the field of the small cell lung cancer tumor marker ProGRP: a method for differentiation of its isoforms.
    Article Snippet: TPCK-treated lyophilized trypsin from bovine pancreas was sequencing grade (Sigma-Aldrich). .. Recombinant ProGRP products, short ProGRP (AA 31−98), ProGRP isoform 1 (AA 1−125 + 8), ProGRP isoform 2 (AA 1−141 + 8), and ProGRP isoform 3 (AA 1−138 + 8), were cloned from human cDNA (OriGene Technologies), expressed in Escherichia coli (Promega) using pGEX-6P-3 constructs (GE Healthcare) and purified as described elsewhere.20 Concentrations of the ProGRP solutions were determined by absorbance at 280 nm (A280) using individually calculated extinction coefficients. ..



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